Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A selection system for identifying accessible sites in target RNAs.

W H Pan1, H F Devlin, C Kelley

  • 1Department of Pathology, Biochemistry and Molecular Biology, The Gittlen Cancer Research Institute, The Pennsylvania State University, Hershey 17033, USA.

RNA (New York, N.Y.)
|May 10, 2001
PubMed
Summary

This study presents a new method to find accessible RNA regions for ribozymes. Ribozymes designed using this method show significantly improved gene silencing activity compared to computer-modeled designs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pacific hotspots reveal a Louisville-Ontong Java Nui tectonic link.

Nature·2025
Same author

Participation effects of workplace promoting activities on healthy eating behavior.

Public health in practice (Oxford, England)·2022
Same author

[Phacoemulsification combined with goniosynechialysis under a gonioscope in the treatment of angle-closure glaucoma: the effect of reopening the synechial anterior chamber angle].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2022
Same author

Effects of socioeconomic status on the control of hypertension in patients <65 and ≥65 years of age in Taiwan: a nationwide cross-sectional study.

BMJ open·2022
Same author

[Analysis of treatment modalities and prognosis of patients with gallbladder cancer in China from 2010 to 2017].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2020
Same author

High sugar-sweetened beverage intake frequency is associated with smoking, irregular meal intake and higher serum uric acid in Taiwanese adolescents.

Journal of nutritional science·2020

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Gene Regulation

Background:

  • Ribozymes offer potential for posttranscriptional gene control.
  • Limited accessibility of target RNA regions hinders ribozyme efficacy.
  • Current design strategies often rely on computer modeling.

Purpose of the Study:

  • To develop a method for identifying accessible RNA regions for oligonucleotide targeting.
  • To improve the catalytic activity of hammerhead ribozymes.
  • To provide a broadly applicable strategy for designing gene-targeting molecules.

Main Methods:

  • A selection protocol to identify accessible RNA regions within transcripts.
  • Construction and testing of hammerhead ribozymes targeted to identified accessible sites.

Related Experiment Videos

  • Comparison of ribozyme activity with computer-modeled designs.

    • Main Results:

    • The developed method successfully identifies accessible RNA regions.
    • Hammerhead ribozymes designed using identified accessible sites exhibit significantly enhanced catalytic activity.
    • This approach outperforms traditional computer modeling for ribozyme design.

    Conclusions:

    • Identifying accessible RNA sites is crucial for effective ribozyme design.
    • The described method enhances ribozyme catalytic activity for gene expression control.
    • This strategy is applicable to designing antisense oligonucleotides and DNAzymes.