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Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum
1College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
Abstract:
Reactive oxygen species (ROS), such as superoxide anions and hydroxyl radicals, are associated with carcinogenesis and other pathophysiological conditions. Therefore, elimination or inactivation of ROS or inhibition of their excess generation may be beneficial in terms of reducing the risk for cancer and other diseases. Ganoderma lucidum has been used in traditional oriental medicine and has potential antiinflammatory and antioxidant activities. In the present study, we tested the amino-polysaccharide fraction (designated as 'G009') from Ganoderma lucidum for the ability to protect against oxidative damage induced by ROS. G009 significantly inhibited iron-induced lipid peroxidation in rat brain homogenates and showed a dose-dependent inactivation of hydroxyl radicals and superoxide anions. It also reduced strand breakage in phiX174 supercoiled DNA caused by UV-induced photolysis of hydrogen peroxide and attenuated phorbol ester-induced generation of superoxide anions in differentiated human promyelocytic leukaemia (HL-60) cells. These findings suggest that G009 from Ganoderma lucidum possesses chemopreventive potential.
Insights
Ganoderma lucidum's G009 fraction demonstrates significant antioxidant and anti-inflammatory properties. This compound effectively neutralizes harmful reactive oxygen species (ROS), suggesting potential for cancer chemoprevention.
Area of Science:
- Biochemistry
- Pharmacology
- Traditional Medicine
Background:
- Reactive oxygen species (ROS) are implicated in cancer development and other diseases.
- Antioxidant interventions targeting ROS may offer protective benefits.
- Ganoderma lucidum is a traditional medicine with known anti-inflammatory and antioxidant potential.
Purpose of the Study:
- To evaluate the antioxidant capacity of the amino-polysaccharide fraction (G009) from Ganoderma lucidum.
- To determine G009's ability to protect against ROS-induced oxidative damage.
- To assess G009's chemopreventive potential.
Main Methods:
- Assessed G009's inhibition of iron-induced lipid peroxidation in rat brain homogenates.
- Measured G009's dose-dependent inactivation of hydroxyl radicals and superoxide anions.
- Evaluated G009's protective effect on DNA strand breakage and ROS generation in HL-60 cells.
Main Results:
- G009 significantly inhibited lipid peroxidation and dose-dependently inactivated hydroxyl radicals and superoxide anions.
- G009 reduced DNA strand breakage induced by UV-activated hydrogen peroxide.
- G009 attenuated superoxide anion generation in human promyelocytic leukemia cells.
Conclusions:
- The amino-polysaccharide fraction G009 from Ganoderma lucidum exhibits potent antioxidant activity.
- G009 effectively scavenges ROS and protects against oxidative damage.
- These findings support the chemopreventive potential of G009 from Ganoderma lucidum.