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CENTA as a chromogenic substrate for studying beta-lactamases
1Centre for Protein Engineering, University of Liège, Institut de Chimie B6, Sart Tilman, 4000 Liège, Belgium.
Antimicrobial Agents and Chemotherapy
|May 17, 2001
Abstract:
CENTA, a chromogenic cephalosporin, is readily hydrolyzed by beta-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of beta-lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.