Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A closed-cycle capillary polymerase chain reaction machine.

J Chiou1, P Matsudaira, A Sonin

  • 1Whitehead Institute, 9 Cambridge Center, Massachusetts 02142, USA.

Analytical Chemistry
|May 17, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Repetitive transcranial magnetic stimulation in the treatment of resistant depression: changes of specific neurotransmitter precursor amino acids.

Journal of neural transmission (Vienna, Austria : 1996)·2021
Same author

Correction to: Large‑scale pathway specific polygenic risk and transcriptomic community network analysis identifies novel functional pathways in Parkinson disease.

Acta neuropathologica·2021
Same author

Built-up area and population density: Two Essential Societal Variables to address climate hazard impact.

Environmental science & policy·2020
Same author

Large-scale pathway specific polygenic risk and transcriptomic community network analysis identifies novel functional pathways in Parkinson disease.

Acta neuropathologica·2020
Same author

SU-E-T-233: Evaluation of Superficial Doses for Postmastectomy Radiotherapy.

Medical physics·2017
Same author

Systematic review: bile acids and intestinal inflammation-luminal aggressors or regulators of mucosal defence?

Alimentary pharmacology & therapeutics·2015
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026
Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026
Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026
Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
See all related articles

A new thermocycling machine uses microcapillary technology for rapid DNA amplification. This device achieved 78% amplification efficiency in 23 minutes, with potential for even faster results.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biophysics

Background:

  • High-speed thermocycling is crucial for accelerating molecular biology workflows.
  • Existing thermocyclers face limitations in speed and efficiency.
  • Microfluidic devices offer potential for miniaturization and rapid thermal cycling.

Purpose of the Study:

  • To describe a novel thermocycling machine utilizing microcapillary technology.
  • To demonstrate the machine's capability for rapid and efficient DNA amplification.
  • To evaluate the theoretical performance limits of the developed system.

Main Methods:

  • A microcapillary system with bidirectional pressure-driven flow and optical position sensors was developed.
  • A 1-microliter reaction mixture droplet was moved between three heat zones using active feedback control.

Related Experiment Videos

  • Scattered light detection was employed for precise droplet positioning and monitoring.
  • Independent adjustment of dwell times and accelerations was implemented.

    • Main Results:

    • The device successfully performed 30 cycles of amplification for a 500-base pair product in 23 minutes.
    • An amplification efficiency of 78% was achieved, comparable to existing high-speed thermocyclers.
    • Theoretical analysis suggests a potential amplification time of 2.5 minutes for 30 cycles.

    Conclusions:

    • The novel microcapillary thermocycler demonstrates significant speed improvements for DNA amplification.
    • The system's design allows for precise control over thermal cycling parameters.
    • This technology holds promise for accelerating molecular diagnostics and research applications.