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Updated: Aug 15, 2026

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
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Does phosphoenolpyruvate carboxykinase have a role in both amino acid and carbohydrate metabolism?
P J Lea1, Z H Chen, R C Leegood
1Department of Biological Sciences, Lancaster University, United Kingdom.
Abstract:
Phosphoenolpyruvate carboxykinase (PEPCK) catalyses the reversible decarboxylation of oxaloacetate to yield phosphoenolpyruvate and CO2. The role of the enzyme in gluconeogenesis and anaplerotic reactions in a range of organisms is discussed, along with the important function in C4 and CAM photosynthesis in higher plants. In addition, new data are presented indicating that PEPCK may play a key role in amino acid metabolism. It is proposed that PEPCK is involved in the conversion of the carbon skeleton of asparagine/aspartate (oxaloacetate) to that of glutamate/glutamine (2-oxoglutarate). This metabolism is particularly important in the transport system, seeds and fruits of higher plants.
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