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Updated: Oct 7, 2026
Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 3, 2015
[Effect of Sisheng Decoction on repairing function of DNA in mice splenic lymphocytes]
1Department of Traditional Chinese Medicine, Changhai Hospital, Shanghai(200433).
Objective:
To explore the mechanism of alleviating the side effects of irradiation by Sisheng Decoction (SSD).
Methods:
The mice splenic lymphocytes were treated by hydroxylurea to stop the DNA replication in S stage, irradiated by ultraviolet ray (UV) and the 3H TdR was added into the cultural medium. After the lymphocytes were cultured in 37 degrees C for 4 h, the capacity for 3H-TdR in the lymphocytes were tested. The effect of SSD in different content on the cut and patch repair(CPR) of DNA can be inferred by observing the lymphocyte uptake index (LUI) of 3H-TdR.
Results:
LUI of mice spleen lymphocytes could be increased by pretreating the lymphocytes with SSD for 4 h and then irradiated by UV, but it could not be increased if the lymphocytes were treated by SSD and UV at the same time.
Conclusion:
SSD can alleviate the side effect of irradiation by enhancing the function of CPR-DNA, which need pretreating the lymphocytes with SSD.