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Outer membrane vesicles from Neisseria meningitidis: effects on tissue factor and plasminogen activator inhibitor-2

M R Mirlashari1, E A Høiby, J Holst

  • 1Research Forum, Ullevaal University Hospital, Oslo, Norway. m.r.mirlashari@ioks.uio.no

Thrombosis Research
|May 23, 2001
PubMed

Insights

Outer membrane vesicles (OMV-LPS) from Neisseria meningitidis increase procoagulant and antifibrinolytic factors in monocytes. This imbalance likely contributes to disseminated intravascular coagulation and organ dysfunction in meningococcal septicemia.

Area of Science:

  • Microbiology
  • Immunology
  • Pathophysiology

Background:

  • Neisseria meningitidis releases outer membrane vesicles (OMV-LPS) during growth.
  • These vesicles play a role in bacterial pathogenesis.

Purpose of the Study:

  • To investigate the effect of OMV-LPS on procoagulant, profibrinolytic, and antifibrinolytic factors in human monocytes.
  • To understand the role of OMV-LPS in the development of severe meningococcal disease.

Main Methods:

  • Purified human monocytes were stimulated with OMV-LPS.
  • Levels of tissue factor, urokinase-type plasminogen activator, and plasminogen activator inhibitor-2 were measured.

Main Results:

  • Cell-associated tissue factor increased 5.0-fold after 8 hours of OMV-LPS exposure.
  • Plasminogen activator inhibitor-2 release increased 13.7-fold after 24 hours of OMV-LPS stimulation.
  • Urokinase-type plasminogen activator levels remained unchanged.

Conclusions:

  • OMV-LPS induces an imbalance favoring fibrin deposition in monocytes.
  • This imbalance is significant in the pathogenesis of disseminated intravascular coagulation and organ dysfunction in meningococcal septicemia.

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