Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

From images to interactions: high-resolution phase imaging in tapping-mode atomic force microscopy.

M Stark1, C Möller, D J Müller

  • 1Max Planck Institute for Biochemistry, Department of Molecular Structural Biology, D-82152 Martinsried, Germany. stark@biochem.mpg.de

Biophysical Journal
|May 24, 2001
PubMed
Summary

Tapping-mode atomic force microscopy reveals material properties using cantilever phase shifts. This technique achieves 1.4-nm resolution, enhancing surface analysis for biological samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prospective external validation of ADNEX and RMI for estimating risk of malignancy in adnexal masses in general gynecological settings: Danish multicenter study.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026
Same author

Greater Thermal Plasticity Toward Heterogeneous Range-Edge Environments of Three <i>Hypericum</i> Species.

Ecology and evolution·2026
Same author

Using Biologics to Reduce Long-Term Corticosteroid Use in Pyoderma Gangrenosum: Real-World Evidence From Two Centres.

The Australasian journal of dermatology·2025
Same author

Artificial Intelligence in the Service of Medicine: Current Solutions and Future Perspectives, Opportunities, and Challenges.

La Clinica terapeutica·2025
Same author

Reduction of brain stem pathology and transient amelioration of early cognitive symptoms in transgenic mice treated with a monoclonal antibody against α-synuclein oligomers/protofibrils.

Aging brain·2023
Same author

The social, psychological, and physical impact of COVID-19 restrictions for institutionalized adults with intellectual and developmental disabilities.

Journal of intellectual disabilities : JOID·2023

Area of Science:

  • Surface science
  • Nanotechnology
  • Biophysics

Background:

  • Tapping-mode atomic force microscopy (AFM) utilizes cantilever phase shifts as a signal.
  • This phase signal provides material-dependent information complementary to surface topography.
  • Understanding the phase signal's origin is crucial for advanced AFM applications.

Purpose of the Study:

  • To demonstrate the localization of information within the phase signal in tapping-mode AFM.
  • To investigate the relationship between the phase signal and tip-sample interactions.
  • To propose a method for extracting interaction information from the phase signal.

Main Methods:

  • Utilized tapping-mode atomic force microscopy (AFM).
  • Analyzed the phase shift between cantilever excitation and response.

Related Experiment Videos

  • Investigated purple membrane of Halobacterium salinarum in buffer solution.
  • Developed a two-order approximation model for phase signal analysis.

    • Main Results:

    • Achieved 1.4-nm lateral resolution in phase signal localization.
    • Demonstrated correlation between phase signal and topography-induced amplitude modulations (first-order approximation).
    • Proposed a method to extract tip-sample interaction information from the phase signal (second-order approximation) for rough surfaces.

    Conclusions:

    • The phase signal in tapping-mode AFM contains localized, material-dependent information.
    • The phase signal is influenced by both topography and tip-sample interactions.
    • The proposed method allows for enhanced characterization of surface properties and interactions.