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Red fluorescent protein (DsRed) as a reporter in Saccharomyces cerevisiae
F Rodrigues1, M van Hemert, H Y Steensma
1Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Journal of Bacteriology
|May 24, 2001
Abstract:
We describe the utilization of a red fluorescent protein (DsRed) as an in vivo marker for Saccharomyces cerevisiae. Clones expressing red and/or green fluorescent proteins with both cytoplasmic and nuclear localization were obtained. A series of vectors are now available which can be used to create amino-terminal (N-terminal) and carboxyl-terminal (C-terminal) fusions with the DsRed protein.