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[Experimental study on endothelialization of autologous pericardial heart valves in vitro]
1Institute of Thoracic and Cardiovascular Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Objective:
To investigate a method of autologous pericardial heart valves (APHV) treatment beneficial to endothelialization.
Methods:
Bovine aortic endothelium cells (BAEC) were cultured and seeded in autologous pericardial pieces treated with different methods in vitro. The influence of different treatment on cellular growing, amount of 3H-TdR incorporation and PGI2 releasing, contents of intracellular cAMP was studied by means of transmission electron microscopy, 3H-TdR incorporation experimentation and radioimmunity method.
Results:
The BAEC seeded in bovine pericardial pieces treated with three different concentrations of GA 10 minutes could alive. In the group with method of treatment with 0.2% GA, the growing of BAEC was best, the cellular density and the amount of 3H-TdR incorporation at different time spots were higher than that of the other groups and it did not enhance the abnormal synthesis of cAMP and PGI2 in BAEC.
Conclusion:
The pericardium treated with 0.2% GA 10 minutes has little toxicity to endothelium cell, which indicates that this method is beneficial to endothelialization of APHV postoperatively.