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Detection of analytes by immunoassay using up-converting phosphor technology
R S Niedbala1, H Feindt, K Kardos
1OraSure Technologies, Inc., 150 Webster Street, Bethlehem, Pennsylvania 18015-1389, USA. sniedbala@orasure.com
Analytical Biochemistry
|May 25, 2001
Summary
Up-Converting Phosphor Technology (UPT) offers sensitive, rapid on-site testing by using lanthanide-based particles that emit visible light when absorbing infrared light. This novel approach eliminates background fluorescence and photobleaching, enabling detection of pathogens and drugs of abuse with high accuracy.
Area of Science:
- Biotechnology
- Nanotechnology
- Analytical Chemistry
Background:
- Up-Converting Phosphor Technology (UPT) utilizes lanthanide-doped ceramic nanoparticles for light emission.
- Biological samples lack up-conversion properties, preventing autofluorescence interference.
- UPT labels are resistant to photobleaching and common assay inhibitors like hemoglobin.
Purpose of the Study:
- To develop and evaluate rapid, on-site diagnostic tests using UPT labels.
- To demonstrate the efficacy of UPT in detecting biological targets and chemical substances.
- To assess the performance of UPT in lateral flow assays compared to existing methods.
Main Methods:
- Development of a UPT reader (UPlink) with a 980 nm infrared laser.
- Implementation of UPT labels in "sandwich" and competitive inhibition assay formats.
- Testing UPT assays for Escherichia coli O157:H7 and a drugs of abuse panel (amphetamine, methamphetamine, phencyclidine, opiates).
Main Results:
- Detection of 10(3) org/mL E. coli O157:H7 in a complex biological background.
- Assay variability (coefficient of variation) below 10% for E. coli detection.
- Multiplexed drug detection with >40% displacement at 10 ng/mL, comparable to EIAs.
- Assay completion times of 10 minutes.
Conclusions:
- UPT enables sensitive and specific on-site testing with minimal background noise.
- UPT-based lateral flow assays offer performance comparable to laboratory methods.
- This technology expands applications for rapid, point-of-care diagnostics.
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