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Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection
G Van den Bosch1, G Locatelli, L Geerts
1Laboratory of Experimental Hematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium.
Journal of Clinical Microbiology
|May 29, 2001
Abstract:
We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease.