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Related Experiment Videos

Dynamic interactions between host and pathogen during acute urinary tract infections.

J D Schilling1, M A Mulvey, S J Hultgren

  • 1Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA.

Urology
|May 30, 2001
PubMed
Summary

Urinary tract infections (UTIs) are complex, involving Escherichia coli invasion and persistence within bladder cells. This intracellular survival evades defenses and antibiotics, potentially leading to recurrent UTIs.

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Mechanisms and consequences of bladder cell invasion by uropathogenic Escherichia coli.

European journal of clinical investigation·2008

Area of Science:

  • Microbiology
  • Immunology
  • Urology

Background:

  • Urinary tract infections (UTIs) traditionally viewed as acute, self-limiting infections caused by noninvasive Escherichia coli.
  • Recent findings reveal complex host-pathogen interactions, including bacterial invasion and persistence, influencing UTI course.
  • The traditional view of UTIs is challenged by evidence of intracellular bacterial behavior.

Purpose of the Study:

  • To investigate the complex interactions between uropathogenic Escherichia coli and bladder epithelial cells.
  • To understand how bacterial invasion and intracellular persistence influence the pathogenesis of UTIs.
  • To explore the mechanisms by which E. coli evades host defenses and antibiotic treatments within the bladder.

Main Methods:

  • Analysis of host-pathogen interactions during acute bladder infection.

Related Experiment Videos

  • Investigation of type 1 pilus-mediated attachment and invasion of bladder epithelial cells by E. coli.
  • Examination of intracellular replication of uropathogenic E. coli within epithelial cells.
  • Assessment of host innate immune responses, including cell death and inflammation.
  • Study of bacterial egress from cells and colonization of surrounding cells.

    • Main Results:

    • E. coli invades bladder epithelial cells, facilitated by type 1 pili, and replicates intracellularly.
    • Intracellular location allows E. coli to evade host defenses and antibiotic therapies.
    • Bacterial invasion and intracellular persistence trigger host inflammatory responses and cell exfoliation.
    • Uropathogenic E. coli can egress from cells to colonize adjacent cells, promoting persistence.
    • E. coli persists in bladder tissue for weeks post-acute infection.

    Conclusions:

    • Acute UTIs involve complex host-pathogen interactions, including bacterial invasion and intracellular persistence.
    • Intracellular E. coli evades host immunity and treatment, contributing to prolonged infection.
    • Bacterial persistence in bladder tissue may underlie chronic conditions like recurrent UTIs and interstitial cystitis.