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Related Experiment Videos

Database-assisted promoter analysis.

R Hehl1, E Wingender

  • 1Institut für Genetik, Technische Universität Braunschweig, Spielmannstr. 7, D-38106,., Braunschweig, Germany. r.hehl@tu-bs.de

Trends in Plant Science
|May 30, 2001
PubMed
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Bioinformatic tools and the TRANSFAC database aid in analyzing DNA regulatory sequences. This approach helps identify transcription factor binding sites for experimental verification of gene regulation.

Area of Science:

  • Bioinformatics
  • Molecular Biology
  • Genomics

Background:

  • Regulatory sequence analysis is crucial for understanding gene expression.
  • Bioinformatic approaches, particularly database utilization, significantly enhance this analysis.
  • The TRANSFAC database is a key resource for transcription factor information.

Purpose of the Study:

  • To highlight the utility of database-assisted bioinformatics for analyzing regulatory DNA sequences.
  • To demonstrate how the TRANSFAC database can identify potential transcription factor binding sites.
  • To outline a workflow for experimental verification of identified regulatory elements.

Main Methods:

  • Utilizing the TRANSFAC database for information on transcription factors, their properties, and binding activities.

Related Experiment Videos

  • Employing software tools to screen a given DNA sequence against the TRANSFAC database.
  • Identifying potential transcription factor binding sites within regulatory sequences.

    • Main Results:

    • Database-assisted screening can identify potential transcription factors that bind to specific DNA sequences.
    • This method facilitates the discovery of functionally relevant regulatory sites.
    • Identified factors and their interactions can be cross-referenced with existing database information.

    Conclusions:

    • Database-assisted bioinformatics, exemplified by TRANSFAC, is an effective strategy for regulatory sequence analysis.
    • This approach streamlines the identification of transcription factor binding sites.
    • It provides a foundation for subsequent experimental validation of regulatory elements and their functions.