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Can 8-oxo-dG be used as a predictor for individual radiosensitivity?
S Haghdoost1, P Svoboda, I Näslund
1Department of Radiotherapy, Radiumhemmet, Stockholm, Sweden. Siamak.haghdoost@ks.se
Summary
Urinary 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxo-dG) may predict acute skin reactions in breast cancer patients undergoing radiation therapy. Lower baseline and during-treatment 8-oxo-dG levels correlate with less severe skin reactions, suggesting its potential as a radiosensitivity marker.
Area of Science:
- Oncology
- Radiation Oncology
- Biomarker Discovery
Background:
- Individual radiosensitivity varies significantly among cancer patients undergoing radiotherapy.
- Predictive tests for radiosensitivity are crucial for optimizing radiation therapy and minimizing side effects.
- Acute skin reactions are common and dose-limiting toxicities in breast cancer patients treated with radiation.
Purpose of the Study:
- To investigate the potential of urinary 7,8-dihydro-8-oxo-2 -deoxyguanosine (8-oxo-dG) as a predictive biomarker for individual radiosensitivity.
- To correlate 8-oxo-dG excretion levels with the severity of acute skin reactions in breast cancer patients receiving adjuvant radiotherapy.
Main Methods:
- Prospective study involving 40 women treated with adjuvant radiotherapy for breast cancer (46 Gy in 2 Gy fractions).
- Clinical scoring of acute skin reactions.
- Quantification of urinary 8-oxo-dG using high-performance liquid chromatography (HPLC) with electrochemical detection before and during radiation treatment.
- Comparison of 8-oxo-dG levels between patients with pronounced skin reactions and those with minor reactions.
Main Results:
- Significantly lower urinary 8-oxo-dG levels (p < 0.001) were observed in patients with pronounced skin reactions compared to those with minor reactions, after normalizing for irradiated volume and dose.
- A significantly lower baseline excretion of 8-oxo-dG (p = 0.043) was found in patients who developed minor skin reactions.
- 8-oxo-dG levels were corrected for body weight and normalized to BMI.
Conclusions:
- Urinary 8-oxo-dG excretion shows potential as a predictive marker for acute radiosensitivity in breast cancer patients.
- This finding could lead to personalized radiation therapy approaches, tailoring treatment based on individual radiosensitivity.
- Further research is warranted to validate 8-oxo-dG as a reliable biomarker for radiosensitivity in broader patient populations.