Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Early Vlambda diversification in sheep.

Y Jeong1, B A Osborne, R A Goldsby

  • 1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01002, USA.

Immunology
|May 31, 2001
PubMed
Summary

The fetal sheep spleen is the earliest site for Vlambda expression and repertoire generation, preceding the development of gut-associated lymphoid tissue. This suggests the spleen plays a key role in early B-cell development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Azimuthal Correlations within Exclusive Dijets with Large Momentum Transfer in Photon-Lead Collisions.

Physical review letters·2023
Same author

Measurement of the mass dependence of the transverse momentum of lepton pairs in Drell-Yan production in proton-proton collisions at <math></math>.

The European physical journal. C, Particles and fields·2023
Same author

Quantitative perfusion and water transport time model from multi b-value diffusion magnetic resonance imaging validated against neutron capture microspheres.

ArXiv·2023
Same author

Measurements of the associated production of a W boson and a charm quark in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2022
Same author

Status of infection prevention and control capacity in Korean hospitals: implications for disaster response and pandemic preparedness.

Public health·2022
Same author

Search for Resonances Decaying to Three W Bosons in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2022

Area of Science:

  • Immunology
  • Developmental Biology
  • Genetics

Background:

  • The development of the antibody repertoire is crucial for adaptive immunity.
  • The ileal Peyer's patch (IPP) is traditionally considered a primary site for B-cell diversification in sheep.
  • Understanding early Vlambda expression sites is key to comprehending immune system development.

Purpose of the Study:

  • To identify the earliest site and timing of Vlambda expression in fetal sheep.
  • To characterize the generation and diversity of the Vlambda repertoire during early gestation.
  • To investigate the role of fetal spleen in early B-cell development.

Main Methods:

  • Collection of tissues (spleen, liver, gut, blood, bone marrow) from ovine fetuses at 48, 55, 60, and 63 gestational days.
  • Preparation of cDNA libraries using reverse transcription-polymerase chain reaction.
  • Sequencing of randomly selected clones and comparison with germline Vlambda genes.

Main Results:

  • Vlambda expression was detected earliest in the spleen (48 gestational days) compared to other examined tissues.
  • The fetal spleen exhibited earlier and higher levels of Vlambda diversity than other tissues.
  • Two germline Vlambda genes (5.1 and 5.3) predominated in early rearrangement, and a novel Jlambda gene was identified.

Conclusions:

  • The fetal spleen is a significant early site for Vlambda repertoire generation, preceding the development of the IPP.
  • The spleen contributes to the diversification of the Vlambda repertoire in sheep.
  • The fetal spleen may provide a partially diversified B-cell repertoire before the IPP becomes a major site for clonal expansion and diversification.

Related Experiment Videos