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Published on: January 12, 2013
Microdialysis can detect age-related differences in glucose distribution within the dermis and subcutaneous adipose
M Boschmann1, F P Murphy, J G Krueger
1German Institute of Human Nutrition, Potsdam-Rehbrücke, Germany. boschman@www.dife.de
Background:
Intrinsic (chronological) cutaneous aging is a continuous and complex process. Limitation in nutrient supply to the skin could contribute to this process.
Objective:
Our study focused on the impact of age on glucose supply to human skin and its distribution within the dermis and subcutaneous adipose tissue.
Methods:
An oral glucose load (100 g) was given to healthy volunteers of two age groups (24 +/- 4 and 59 +/- 10 years, n = 4 for each group). Dialysate concentrations of glucose and glycerol were monitored by microdialysis in the dermis and adipose tissue.
Results:
Baseline tissue [glucose] did not differ significantly between the two groups. After the glucose load, dialysate [glucose] of the dermis peaked between 60 and 80 min and between 80 and 120 min in the young and old groups, respectively. In the old group, dialysate [glucose] was significantly higher in the dermis than adipose tissue at 80, 100, 120, 160 and 180 min after the load. The sum of the areas under the curve (dermis + adipose tissue) did not differ significantly between the two age groups.
Conclusions:
Aging is accompanied by an increased diffusion barrier for glucose between the dermis and adipose tissue.
