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Dissection of defence response pathways in rice
1Department of Plant Pathology, 4024 Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.
Novartis Foundation Symposium
|June 5, 2001
Summary
Researchers are identifying rice mutants with altered defence response (DR) genes to understand plant disease resistance. This work aids in discovering new genes involved in plant immunity and metabolic pathways.
Area of Science:
- Plant molecular biology
- Genetics
- Crop science
Background:
- Major resistance genes offer insights into plant immunity induction.
- Downstream defence response (DR) genes collectively confer resistance, but their roles are not fully understood.
Purpose of the Study:
- To dissect rice resistance pathways by identifying mutants with altered DR genes.
- To establish a collection of enhanced sequence tags (ESTs) and putative DR genes for mutant detection.
Main Methods:
- Screening rice mutants for altered resistance/susceptibility to Magnaporthe grisea and Xanthomonas oryzae pv. oryzae.
- Utilizing ESTs and putative DR genes to detect mutants with deletions.
- Employing DR gene arrays to generate gene expression profiles of selected mutants.
Main Results:
- A collection of rice mutants with structural rearrangements in DR genes has been generated.
- A library of ESTs and putative DR genes is available for mutant screening.
- Gene expression profiling of mutants is planned to identify genes involved in disease response.
Conclusions:
- The mutant screen is a valuable tool for identifying candidate genes in plant disease response.
- This approach will advance understanding of rice immunity and other metabolic pathways.