Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rolipram reduces excitotoxic neuronal damage.

F Block1, W Schmidt, M Nolden-Koch

  • 1Department of Neurology RWTH Aachen, Germany.

Neuroreport
|June 5, 2001
PubMed
Summary

Rolipram, a tumor necrosis factor-alpha inhibitor, reduced excitotoxic neuronal damage in rats when administered early after quinolinic acid injection. This suggests a potential therapeutic role for TNF-alpha inhibition in preventing brain injury.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Constraints on General Neutrino Interactions Based on KATRIN Data.

Physical review letters·2025
Same author

New Constraint on the Local Relic Neutrino Background Overdensity with the First KATRIN Data Runs.

Physical review letters·2022
Same author

Bound on 3+1 Active-Sterile Neutrino Mixing from the First Four-Week Science Run of KATRIN.

Physical review letters·2021
Same author

Improved Upper Limit on the Neutrino Mass from a Direct Kinematic Method by KATRIN.

Physical review letters·2019
Same author

[Availability of mechanical thrombectomy for acute stroke : Analysis of the health care reality in Germany].

Der Nervenarzt·2017
Same author

The DeepTree Exhibit: Visualizing the Tree of Life to Facilitate Informal Learning.

IEEE transactions on visualization and computer graphics·2015

Area of Science:

  • Neuroscience
  • Neuroinflammation
  • Pharmacology

Background:

  • Proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), are implicated in neuronal damage following excitotoxic injuries.
  • Understanding the role of TNF-α in excitotoxicity is crucial for developing neuroprotective strategies.

Purpose of the Study:

  • To investigate the neuroprotective effects of rolipram, a selective TNF-α inhibitor, against excitotoxic neuronal damage induced by quinolinic acid (QA).

Main Methods:

  • Stereotactic injection of quinolinic acid into the striatum of male Wistar rats to induce excitotoxic lesions.
  • Administration of rolipram (0.3 mg/kg) or MK-801 (a positive control) at different time points (6h and 24h post-QA) or solvent.
  • Assessment of striatal neuronal damage after 14 days of daily treatment.

Related Experiment Videos

Main Results:

  • Quinolinic acid injection caused extensive neuronal damage (93% of striatal area).
  • MK-801 significantly reduced damage to 12%.
  • Rolipram treatment initiated 6 hours after QA injection reduced damage to 60%, while treatment initiated 24 hours post-QA showed no significant difference from the solvent group (91% damage).

Conclusions:

  • Early administration of rolipram demonstrates significant neuroprotective effects against quinolinic acid-induced excitotoxicity.
  • These findings highlight the potential of targeting TNF-α pathways for mitigating neuronal damage in excitotoxic conditions.