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Related Experiment Videos

A novel microphysiometer based on MLAPS for drugs screening.

W Yicong1, W Ping, Y Xuesong

  • 1Biosensor National Special Lab., Dept. of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, People's Republic of China.

Biosensors & Bioelectronics
|June 8, 2001
PubMed
Summary

This study introduces a new microphysiometer for measuring cell ion concentrations. This device analyzes drug effects on cell metabolism and receptor responses in real-time.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Pharmacology

Background:

  • Accurate measurement of extracellular ion concentrations is crucial for understanding cell function and drug responses.
  • Existing methods may lack the real-time, multi-ion measurement capabilities needed for comprehensive cellular analysis.

Purpose of the Study:

  • To develop and validate a novel microphysiometer for simultaneous, real-time measurement of extracellular ions (H+, Na+, K+, Ca2+) in living cells.
  • To investigate the effects of various Western and Chinese பாரம்பரிய medicines on cellular ion dynamics and metabolism.
  • To establish a platform for rapid drug evaluation and dose-response curve generation under standard cell culture conditions.

Main Methods:

  • Utilizing a multi-light addressable potentiometric sensor (MLAPS) based microphysiometer.

Related Experiment Videos

  • Employing parallel processing algorithms to analyze on-line measurements of response amplitudes from illuminated sensitive membranes at different frequencies.
  • Conducting experiments with living cells exposed to specific drugs (dilantin, phenobarbital sodium, penicillin sodium, scutellaria, medlar, hemlock parsley).

    • Main Results:

    • Demonstrated simultaneous measurement of extracellular H+, Na+, K+, and Ca2+ concentrations.
    • Analyzed the relationship between drug administration and changes in extracellular ion profiles.
    • Successfully estimated the effects of tested Western and Chinese drugs on cellular ion balance and metabolism.

    Conclusions:

    • The novel microphysiometer enables real-time monitoring of cell metabolism and receptor function through ion detection.
    • The system facilitates efficient drug evaluation, including dose-response assessments, within hours.
    • This technology offers a valuable tool for pharmacological research and drug development by providing insights into cellular responses.