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Direct resolution of (+/-)-ephedrine and atropine into their enantiomers by impregnated TLC
R Bhushan1, J Martens, M Arora
1Department of Chemistry, University of Roorkee, Roorkee 247 667, India.
Abstract:
Direct resolution of (+/-)-ephedrine and atropine into their enantiomers was achieved by normal-phase thin layer chromatography on silica gel plates impregnated with optically pure L-tartaric acid and L-histidine, respectively, as chiral selectors. The mobile phases enabling successful resolution were different combinations of acetonitrile-methanol-water. The spots were detected with iodine vapours and the detection limits were 2 and 6 microg, respectively, in terms of the racemate. The effects of concentration of the impregnating reagent, temperature and pH on resolution have been studied.
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