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Molecularly imprinted polymers from nicotinamide and its positional isomers
1Laboratory Bioorgan and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
Journal of Molecular Recognition : JMR
|June 8, 2001
Summary
Molecular imprinting polymers were developed for nicotinamide and its isomers. These polymers show selective binding due to synergistic effects, enabling separation of positional isomers via HPLC.
Area of Science:
- Polymer Chemistry
- Analytical Chemistry
- Separation Science
Background:
- Molecular imprinting polymers (MIPs) are crucial for selective molecular recognition.
- Understanding the influence of polymer composition on recognition is key for developing selective materials.
- Nicotinamide and its isomers present a challenge for selective separation due to structural similarities.
Purpose of the Study:
- To prepare imprinted polymers for nicotinamide and its positional isomers.
- To investigate the impact of porogenic solvent and functional monomer on polymer recognition properties.
- To evaluate the polymers as stationary phases for High-Performance Liquid Chromatography (HPLC) separation.
Main Methods:
- Synthesis of imprinted polymers using nicotinamide, isonicotinamide, and picolinamide as templates.
- Evaluation of polymer recognition properties based on binding affinities.
- Application of synthesized polymers as HPLC stationary phases for isomer separation.
Main Results:
- Polymers imprinted with nicotinamide and isonicotinamide exhibited synergistic binding effects, enhancing recognition.
- These polymers successfully separated positional isomers of nicotinamide and isonicotinamide using HPLC.
- Polymers prepared with picolinamide showed no specific recognition for the template.
- The polymers demonstrated dual recognition, also retaining structurally similar compounds like nicotinic acid.
Conclusions:
- The heterocyclic nitrogen and amide groups contribute synergistically to the binding of nicotinamide and isonicotinamide to imprinted polymers.
- MIPs synthesized with nicotinamide and isonicotinamide are effective HPLC stationary phases for separating positional isomers.
- The dual recognition capability of these polymers offers an advantage for applications with challenging template stability or solubility.
- The study elucidates the mechanisms behind differential recognition in MIPs for closely related molecules.