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2-Chloroacetaldehyde induces epsilondA DNA adducts in DNA of Raji cells as demonstrated by an improved
1Div. Genetic Alterations in Carcinogenesis C0700, DKFZ Heidelberg, Germany.
Fresenius' Journal of Analytical Chemistry
|June 8, 2001
Abstract:
Etheno adducts in DNA are promutagenic lesions and are formed from vinyl chloride, urethane, and lipid peroxidation-derived products such as 4-hydroxynonenal. Such adducts were induced in a B-lymphoid cell line (Raji) by a metabolite of vinyl chloride, 2-chloroacetaldehyde. By modification of a method of Bedell et al., etheno dA was determined in 20 microg DNA by HPLC-fluorimetry. Our method has a detection limit of 5 fmol which is one two-hundredth that of the original method. By use of this method initial evidence was found that cigarette smoke can also induce etheno dA adducts in Raji cell DNA.