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An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate
S Teotia1, S K. Khare, M N. Gupta
1Chemistry Department, Indian Institute of Technology, Hauz Khas, 110016, New Delhi, India
Enzyme and Microbial Technology
|June 9, 2001
Abstract:
beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of beta-amylases.