Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of multicomponent monosaccharide solutions using an enzyme-based sensor array.

T E Curey1, A Goodey, A Tsao

  • 1Department of Chemistry & Biochemistry, University of Texas, Austin, Texas 78712, USA.

Analytical Biochemistry
|June 12, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A GPS-based investigation into the relationship between exercise irregularity and osteochondritis dissecans in Thoroughbred weanlings: A case-control study.

Veterinary journal (London, England : 1997)·2025
Same author

The Link between Hyperinsulinemia and Periodontitis in Diabetics.

Journal of dental research·2025
Same author

Racing performance in 75 Thoroughbreds after arthroscopic removal of Osteochondritis dissecans from the lateral femoral trochlear ridge before first race start in Korea (2015-2017).

Veterinary journal (London, England : 1997)·2025
Same author

Effect of Various Toothpaste Tablets on Gloss and Surface Roughness of Resin-based Composite Materials.

Operative dentistry·2024
Same author

Good Practices for the Laboratory Performance Testing of Aqueous Oral Inhaled Products (OIPs): an Assessment from the International Pharmaceutical Aerosol Consortium on Regulation and Science (IPAC-RS).

AAPS PharmSciTech·2023
Same author

Nuclear F-actin Cytology in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma.

Journal of dental research·2020
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

This study presents a novel sensor for simultaneous multi-sugar measurement in aqueous solutions. The micromachined silicon chip, using enzyme-based assays, accurately quantifies glucose and galactose with no cross-talk.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biosensor Technology

Background:

  • Accurate and simultaneous measurement of multiple sugars is crucial for various applications, including clinical diagnostics and food analysis.
  • Existing methods often face limitations in speed, sensitivity, or multiplexing capabilities.
  • Enzyme-based assays offer high specificity but require precise immobilization and detection strategies.

Purpose of the Study:

  • To develop a novel sensor for rapid, simultaneous quantification of multiple sugars in aqueous samples.
  • To demonstrate the sensor's capability for accurate sugar measurement at low micromolar to millimolar concentrations.
  • To showcase the sensor's potential for integrated analysis and signal normalization.

Main Methods:

  • Development of a micromachined silicon chip with an array of individually addressable sites for enzyme-based assays.

Related Experiment Videos

  • Localization of microspheres derivatized with monosaccharide-specific dehydrogenases within pyramidal silicon cavities.
  • Utilizing a liquid chromatography pumping system for sample delivery and stopped-flow conditions for fluorescence monitoring.
  • Analysis via direct fluorescence detection of reduced nicotinamide cofactors (NADH, NADPH) or secondary fluorescent products.

    • Main Results:

    • Successful quantification of beta-D-glucose and beta-D-galactose at low micromolar to millimolar levels.
    • Demonstrated absence of detectable cross-talk between different assay sites on the sensor array.
    • Validated the sensor's ability to normalize fluorescence signals using integrated glucose and pH measurements.
    • Successfully applied standard additions for serum glucose determination, accounting for enzyme activity variations.

    Conclusions:

    • The developed sensor enables rapid and simultaneous measurement of multiple sugars with high accuracy and specificity.
    • The array format offers redundancy, potential for multiplexing, and integrated control of assay conditions.
    • This technology holds promise for advanced diagnostics, environmental monitoring, and process control applications requiring precise sugar analysis.