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Related Experiment Videos

Multiple responses in gene expression in fish treated with estrogen.

N D Denslow1, H S Lee, C J Bowman

  • 1Department of Biochemistry and Molecular Biology, University of Florida, PO Box 100156 HC, 32610, Gainesville, FL, USA. denslow@biotech.ufl.edu

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
|June 12, 2001
PubMed
Summary

Environmental estrogens, like industrial chemicals, alter gene expression in male sheepshead minnows. These changes, including vitellogenin up-regulation, serve as biomarkers for estrogenic exposure and its effects.

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Area of Science:

  • Environmental Science
  • Molecular Biology
  • Toxicology

Background:

  • Environmental estrogens are chemicals that mimic natural estrogen, potentially disrupting biological processes.
  • Estrogen receptors play a crucial role in regulating gene expression, influencing development and reproduction.
  • Previous research indicates environmental estrogens can induce biological changes, primarily through altered gene expression.

Purpose of the Study:

  • To investigate and map qualitative gene expression responses in male sheepshead minnows exposed to estradiol-17beta.
  • To determine if different exposure methods (injection vs. aquatic) yield similar gene expression patterns.
  • To identify specific genes affected by environmental estrogen exposure as potential biomarkers.

Main Methods:

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  • Adult male sheepshead minnows (Cyprinidon variegatus) were used for the study.
  • Fish were exposed to high-dose injections (5 mg/kg) or environmentally relevant concentrations (100 ng/l) of estradiol-17beta.
  • Differential display reverse transcriptase polymerase chain reaction (DD) was employed to analyze gene expression changes.

    • Main Results:

    • Gene expression responses to both high-dose injections and low-dose aquatic exposures were nearly identical.
    • Both up-regulation and down-regulation of transcripts were observed, consistent with known estradiol responses.
    • Genes such as vitellogenin and vitelline envelope proteins were up-regulated, indicating susceptibility of egg development-related genes.

    Conclusions:

    • Environmental estrogen exposure significantly alters gene expression in male fish.
    • Identified gene expression changes, particularly vitellogenin, can serve as reliable "fingerprints" for in vivo estrogenic responses.
    • These findings highlight the impact of environmental estrogens on reproductive-related gene expression.