Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adenovirus-mediated BMP2 expression in human bone marrow stromal cells.

E A Olmsted1, J S Blum, D Rill

  • 1Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA.

Journal of Cellular Biochemistry
|June 16, 2001
PubMed
Summary

Recombinant adenoviruses can deliver bone morphogenetic protein 2 (BMP2) genes to cells. BMP2 production in bone marrow stromal cells depends on their differentiation state and specific culture conditions for effective bone regeneration therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Report of Podosphaera xanthii Race 1W Causing Powdery Mildew of Watermelon in California.

Plant disease·2019
Same author

Estrogen receptors in breast and bone: from virtue of remodeling to vileness of metastasis.

Oncogene·2017
Same author

Physical Characterization and Innate Immunogenicity of Aggregated Intravenous Immunoglobulin (IGIV) in an In Vitro Cell-Based Model.

Pharmaceutical research·2016
Same author

The prevalence of alcohol misuse in patients undergoing cataract surgery.

Addiction biology·2016
Same author

Response of the hairy mussel Trichomya hirsuta to sediment-metal contamination in the presence of a bioturbator.

Marine pollution bulletin·2014
Same author

Is nectar reabsorption restricted by the stalk cells of floral and extrafloral nectary trichomes?

Plant biology (Stuttgart, Germany)·2014

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Orthopedic Research

Background:

  • Recombinant adenoviral vectors show promise for treating musculoskeletal defects.
  • Developing effective bone production systems is a key focus in orthopedic research.

Purpose of the Study:

  • To determine optimal conditions for sustained, high-level active bone morphogenetic protein 2 (BMP2) production using adenovirus type 5 (Ad5BMP2).
  • To evaluate the impact of Ad5BMP2 infection on osteoprogenitor cells and BMP2 synthesis.

Main Methods:

  • Utilized recombinant adenovirus type 5 (Ad5BMP2) for gene delivery.
  • Infected various cell lines, including A549, human bone marrow stromal cells, and mouse bone marrow stromal cell line W20-17.
  • Manipulated cell culture conditions, including dexamethasone and ascorbic acid treatment, and cell confluency.

Related Experiment Videos

Main Results:

  • Achieved high levels (144 ng/ml) of biologically active BMP2 in A549 cells.
  • Observed significantly lower BMP2 expression in undifferentiated human and mouse bone marrow stromal cells.
  • Demonstrated that differentiated human bone marrow stromal cells, particularly osteoblast-like cells, produced high BMP2 levels upon Ad5BMP2 infection.
  • Found that BMP2 production was dependent on the differentiation state of stromal cells and specific culture conditions, with confluency and timing of Ad5BMP2 administration impacting expression.

Conclusions:

  • Adenovirus-mediated BMP2 expression in bone marrow stromal cells is highly dependent on the cells' differentiation status.
  • Optimized culture conditions, including specific treatments and avoiding confluency, are crucial for efficient BMP2 production for potential bone regeneration therapies.