Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Caveolin and Ras function.

R G Parton1, J F Hancock

  • 1Centre for Microscopy and Microanalysis, Department of Physiology and Pharmacology, and Institute of Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia.

Methods in Enzymology
|June 13, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Biology of Endosomal Escape: Strategies for Enhanced Delivery of Therapeutics.

ACS nano·2026
Same author

Targeted glycophagy ATG8 therapy reverses diabetic heart disease in mice and in human engineered cardiac tissues.

bioRxiv : the preprint server for biology·2025
Same author

Targeted glycophagy ATG8 therapy reverses diabetic heart disease in mice and in human engineered cardiac tissues.

Nature cardiovascular research·2025
Same author

An anaplerotic approach to correct the mitochondrial dysfunction in ataxia-telangiectasia (A-T).

Molecular metabolism·2021
Same author

Evidence of epistatic suppression of repeat fruiting in cultivated strawberry.

BMC plant biology·2019
Same author

A microtubule-organizing center directing intracellular transport in the early mouse embryo.

Science (New York, N.Y.)·2017
Same journal

1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

Methods in enzymology·2026
Same journal

Nitrone dipoles in bioorthogonal chemistry applications.

Methods in enzymology·2026
Same journal

Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

Methods in enzymology·2026
Same journal

Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

Methods in enzymology·2026
Same journal

inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

Methods in enzymology·2026
Same journal

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026
See all related articles

New experimental protocols enable precise localization of signaling proteins, like Ras proteins, to specific plasma membrane subdomains. This is crucial for understanding signal transduction and differential signaling outputs from homologous proteins.

Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Understanding signal transduction requires precise localization of signaling proteins within the plasma membrane.
  • Ras proteins, key signaling molecules, are directed to distinct membrane microdomains by unique anchors.
  • These microdomains influence Ras protein function and downstream signaling outcomes.

Purpose of the Study:

  • To present experimental protocols for confident assignment of signaling proteins to specific plasma membrane subdomains.
  • To highlight the utility of these methods for studying Ras protein function and localization.
  • To demonstrate the broad applicability of these techniques in mammalian signal transduction research.

Main Methods:

  • Development of novel experimental protocols.

Related Experiment Videos

  • Techniques for protein localization studies.
  • Methods for analyzing plasma membrane microdomains.

    • Main Results:

    • Protocols allow confident assignment of signaling proteins to specific membrane subdomains.
    • Demonstrated utility in studying Ras protein targeting and function.
    • Methods show wide applicability across various signal transduction pathways.

    Conclusions:

    • The presented experimental protocols are essential for understanding signal transduction.
    • These methods provide critical insights into Ras protein localization and function.
    • The techniques have broad utility for studying mammalian signal transduction pathways.