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High-throughput microfabricated CE/ESI-MS: automated sampling from a microwell plate
1Barnett Institute and Department of Chemistry, Northeastern University, Boston, Massachusetts 02115, USA.
Analytical Chemistry
|June 14, 2001
Summary
This study introduces a novel microfabricated capillary electrophoresis/electrospray mass spectrometry (CE/ESI-MS) system for high-throughput automated peptide and protein digest analysis. The innovative design simplifies chip fabrication and enables extended operation for efficient sample processing.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Microfluidics
Background:
- High-throughput analysis of peptides and protein digests is crucial for proteomics.
- Existing capillary electrophoresis/electrospray mass spectrometry (CE/ESI-MS) systems can be complex to fabricate and operate.
Purpose of the Study:
- To present a new design for a microfabricated CE/ESI-MS system with automated sampling.
- To simplify the chip design and enable extended operation for high-throughput analysis.
Main Methods:
- Developed a microfabricated device integrating sample loading, separation channel, and liquid junction for CE/ESI-MS.
- Utilized a polycarbonate manifold with external reservoirs for electrokinetic and pressure-fluid control.
- Employed a computer-activated electropneumatic distributor for automated sample loading and channel washing.
Main Results:
- Demonstrated a simplified microchip design by externalizing electrodes and sample reservoirs.
- Enabled extended system operation through the use of larger external reservoirs.
- Presented initial results for automated CE/ESI-MS analysis of peptides and protein digests.
Conclusions:
- The new microfabricated CE/ESI-MS system offers a simplified design for high-throughput automated analysis.
- The external manifold approach facilitates extended operation and efficient sample processing.
- This technology holds promise for advancing proteomics research through improved analytical capabilities.