Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aromatase gene expression in the stallion.

E Lemazurier1, P Sourdaine, C Nativelle

  • 1Laboratoire de Biochimie et Biologie Moléculaire, IBBA EA 2608, Université de Caen, Esplanade de la Paix, 14032 cedex, Caen, France.

Molecular and Cellular Endocrinology
|June 19, 2001
PubMed
Summary

The adult stallion

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determination of endocrine disruptors and endogenic androgens and estrogens in rat serum by high-performance liquid chromatography-tandem mass spectrometry.

Steroids·2015
Same author

Cell-cell interactions in the testis of the dogfish: stage-related changes in protein synthesis.

Fish physiology and biochemistry·2013
Same author

Expression of a natural antisense transcript of Cg-Foxl2 during the gonadic differentiation of the oyster Crassostrea gigas: first demonstration in the gonads of a lophotrochozoa species.

Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation·2012
Same author

Identification of Ras, Pten and p70S6K homologs in the Pacific oyster Crassostrea gigas and diet control of insulin pathway.

General and comparative endocrinology·2011
Same author

An engineered 3D blood-testis barrier model for the assessment of reproductive toxicity potential.

Biomaterials·2010
Same author

Dynamic respiratory endoscopy without treadmill in 68 performance Standardbred, Thoroughbred and saddle horses under natural training conditions.

Equine veterinary journal·2009

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Reproductive Science

Background:

  • Adult stallions exhibit high estrogen levels, primarily linked to cytochrome P450 aromatase (P450 arom) in Leydig cells.
  • Understanding aromatase distribution is crucial for explaining equine estrogen synthesis and potential neurosteroid roles.

Purpose of the Study:

  • To investigate the detailed distribution and expression of aromatase in various adult stallion tissues.
  • To correlate aromatase levels with estrogen synthesis and explore its potential role in brain function.

Main Methods:

  • Quantitative RT-PCR to detect aromatase mRNA expression across tissues.
  • Enzyme-linked immunosorbent assay (ELISA) and immunoblotting to quantify and identify aromatase protein.
  • Enzyme activity assays using radiolabeled androstenedione to measure aromatase function.

Main Results:

  • Aromatase mRNA, protein, and activity were significantly higher in stallion testes compared to brain, liver, kidney, intestine, muscle, and bulbourethral glands.
  • The brain showed detectable levels of aromatase mRNA, protein, and activity, suggesting a potential neurosteroid role.
  • Lower aromatase levels in a colt's testis indicated developmental regulation and potential as a marker for testicular function.

Conclusions:

  • The stallion testis is the primary source of aromatase, responsible for high estrogen synthesis.
  • The brain's aromatase expression suggests a role for neurosteroids in stallion behavior and endocrine control.
  • Aromatase expression is developmentally regulated in stallions, offering a potential marker for testicular health.

Related Experiment Videos