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Related Experiment Videos

Comparison between the quality and function of sperm after semen processing with two different methods.

M E Hammadeh1, P M Zavos, P Rosenbaum

  • 1Dept of Obs/Gyn, University of Saarland, Homburg/Saar, Germany. frmham@med-rz.uni-sb.de

Asian Journal of Andrology
|June 19, 2001
PubMed
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Percoll gradient centrifugation better selects for sperm with normal chromatin and morphology compared to SpermPrep filtration columns, though SpermPrep offers higher sperm recovery rates. Fertilization rates were similar between methods.

Area of Science:

  • Reproductive Science
  • Andrology
  • In Vitro Fertilization

Background:

  • Semen processing is crucial for assisted reproductive technologies (ART).
  • SpermPrep filtration columns and Percoll gradient centrifugation are common methods for sperm selection.
  • Evaluating sperm morphology and chromatin condensation is vital for ART success.

Purpose of the Study:

  • To compare SpermPrep and Percoll in recovering morphologically normal and chromatin-condensed spermatozoa.
  • To assess the impact of these processing methods on fertilization and pregnancy rates in IVF-ET.

Main Methods:

  • Sixteen semen samples were divided and processed using either SpermPrep or Percoll gradient centrifugation.
  • Sperm morphology (strict criteria) and chromatin condensation (CMA3) were evaluated pre- and post-processing.

Related Experiment Videos

  • Oocytes were inseminated with spermatozoa processed by each method to determine fertilization rates.

    • Main Results:

    • Percoll yielded significantly higher percentages of chromatin-condensed (90.8%) and morphologically normal (12.9%) spermatozoa compared to SpermPrep (82.3% and 6.9%, respectively).
    • SpermPrep demonstrated a significantly higher sperm count recovery rate than Percoll.
    • Fertilization rates were comparable between the two semen processing techniques.

    Conclusions:

    • Percoll gradient centrifugation is recommended for procedures like intracytoplasmic sperm injection (ICSI) due to enhanced sperm selection.
    • SpermPrep is suggested for IVF and IUI where sperm concentration is a more critical factor.
    • The choice of method depends on the specific ART procedure and desired sperm characteristics.