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Updated: Aug 8, 2026
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Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Published on: March 13, 2015
Simultaneous measurement of antibodies to three HIV-1 antigens in newborn dried blood-spot specimens using a
R Bellisario1, R J Colinas, K A Pass
1Division of Genetic Disorders, Wadsworth Center, New York State Department of Health, 12201-0509, Albany, NY, USA. bellisar@wadsworth.org
Early Human Development
|June 16, 2001
Abstract:
We developed a fluorescent immunoassay to simultaneously measure antibodies to three HIV-1 antigens from newborn dried blood-spot specimens. The multiplexed assay uses fluorescent microspheres and a flow analyzer. The procedure is sensitive, precise and accurate, and can be expanded to simultaneously measure additional multiple analytes from a single specimen.
