[Studies on eicosapentaenoic acid production by submersion culture of Cryphecodinium cohnii]

The effects of the incubation temperature, initial pH of the medium, carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied. Through orthogonal experiments, the optimum culture medium was obtained (g/L): Glucose, 65; Yeast extract, 2.0; KNO3 3.0, KH2PO4 1.0, MgSO4.7H2O 0.6, NaCl 5.8, CaCl2.2H2O 0.1, ZnSO4.7H2O 0.0067, FeCl3.6H2O 0.014, CuSO4.5H2O 0.0004, MnSO4.H2O 0.0002. Under the optimum culture conditions, the microalgae dry cell weight and eicosapentaenoic acid was 18.3 g/L and 0.891 g/L, respectively. The submersion culture process was analysed.