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Fluorescence spectroscopic analysis of circulating platelet activation during coronary angioplasty
A Christov1, W J Kostuk, G Jablonsky
1Vascular Biology Group, John P. Robarts Research Institute, University of Western Ontario, London, Ontario N6A 5K8, Canada.
Background And Objective:
Platelet activation during percutaneous transluminal coronary angioplasty (PTCA) initiates thrombus formation and plaque regrowth at sites of arterial injury, limiting procedure efficacy. We have developed a simple assay for circulating platelet activation based on fluorescence analysis of membrane fluidity and intracellular calcium concentration and light scattering analysis of platelet aggregation.
Study Design/Materials And Methods:
Platelet activation state was measured in 45 patients undergoing angioplasty, before and after treatment with platelet inhibitors.
Results:
PTCA alone produced a decrease in pyrene dimer formation (P0.0083) and an increase in light scattering at 650 nm (P0.0128). Treatment with ADP and GPIIb/IIIa receptor antagonists reduced PTCA induced changes in pyrene dimer formation. An unexpected decrease in pyrene dimer formation (P0.05) was detected when the GPIIb/IIIa receptor antagonist was given together with an ADP receptor antagonist.
Conclusions:
1) Analysis of membrane fluidity provides a sensitive marker for platelet activation state. 2) Reduced membrane fluidity after combined platelet inhibitor treatments suggests reduced antiplatelet efficacy.