Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Silver ion microplates for immunoassays.

M R Bonen1, S A Hoffman, A A García

  • 1Arizona State University, Tempe, AZ, USA.

Biotechniques
|June 21, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a test-and-cull strategy to control bovine paratuberculosis based on the detection of Mycobacterium avium ssp. paratuberculosis DNA using the high-throughput Johne's fecal polymerase chain reaction test.

Journal of dairy science·2025
Same author

Microstructure response of concentrated suspensions to flow reversal.

The European physical journal. E, Soft matter·2025
Same author

Relationship between internal medicine journals' activity on social networks and the citations they receive.

Revista clinica espanola·2021
Same author

Purified Human Pancreatic Islets, CIT Culture Media with Lisofylline or Exenatide.

CellR4-- repair, replacement, regeneration, & reprogramming·2019
Same author

A multicenter, open-label, expanded phase 2 study to evaluate the safety and efficacy of etirinotecan pegol, a polymer conjugate of irinotecan, in women with recurrent platinum-resistant or refractory ovarian cancer.

Gynecologic oncology·2017
Same author

Allergic contact dermatitis to silver in a patient treated with silver sulphadiazine after a burn.

Journal of the European Academy of Dermatology and Venereology : JEADV·2014

Silver ions in microplate wells effectively immobilize biotin-labeled proteins like horseradish peroxidase (HRP) and antibodies, enabling sensitive detection. This method enhances bioactivity and reduces background noise for improved assays.

Area of Science:

  • Biochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Surface modification of microplates is crucial for immobilizing biomolecules.
  • Developing stable and active immobilization methods is essential for sensitive immunoassays.

Purpose of the Study:

  • To develop a novel method for immobilizing biotin-labeled proteins using silver ions on microplate surfaces.
  • To evaluate the efficiency and bioactivity of immobilized proteins and antibodies.
  • To determine the sensitivity and specificity of assays utilizing these modified microplates.

Main Methods:

  • Microplate wells were coated with silver ions using glutaraldehyde and thiourea.
  • Biotin-labeled horseradish peroxidase (HRP) and antibodies were immobilized onto silver ion-modified surfaces.

Related Experiment Videos

  • Surface coverage, binding constants, and detection limits were determined.
  • Bioactivity of immobilized antibodies was assessed.

    • Main Results:

    • Silver ions facilitated the immobilization of active biotin-labeled HRP and antibodies.
    • Maximum surface coverage for biotin-HRP was 40 ng/cm², with a binding constant (Km) of 8 x 10^9 M.
    • Detection of as little as 6.7 fmol of HRP was achieved.
    • Low background binding was observed, enabling sensitive detection (10^-14 to 10^-11 mol HRP).

    Conclusions:

    • Silver ion-modified microplates provide an effective platform for immobilizing bioactive proteins.
    • This method offers high sensitivity and low background for biochemical assays.
    • The approach is suitable for developing advanced diagnostic and detection systems.