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Integrin expression at the bone/biomaterial interface.

S A Clarke1, P A Revell

  • 1Orthopaedic Research Unit, Box 180, Addenbrooke's Hospital, Hill's Road, Cambridge, CB2 2QQ, United Kingdom. sac42@cam.ac.uk

Journal of Biomedical Materials Research
|June 21, 2001
PubMed
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This study visualized cell integrin expression in joint replacement interface tissue. Macrophages likely use beta-2 integrins (CD11a, CD11b) for transmigration, indicated by specific adhesion molecule expression.

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Cell Biology

Background:

  • Aseptically loose total joint replacements present a challenge in orthopedic surgery.
  • Understanding cellular interactions within the interface tissue is crucial for addressing implant loosening.
  • Integrins play vital roles in cell adhesion and migration, influencing tissue responses.

Purpose of the Study:

  • To visualize and characterize integrin expression on cells within the interface tissue of aseptically loose total joint replacements.
  • To correlate integrin expression with the presence of their specific ligands in the same tissue.
  • To elucidate the potential role of specific integrins in cellular transmigration within this context.

Main Methods:

  • Tissue samples from 25 patients undergoing revision arthroplasty were analyzed.

Related Experiment Videos

  • Immunolabeling was performed for multiple integrins (e.g., alpha(2)beta(1), alpha(v)beta(3), alpha(4)beta(1), CD11a, CD11b, CD11c) and their ligands (e.g., fibronectin, laminin, vitronectin, ICAM-1, VCAM-1).
  • Expression patterns of integrins and ligands were examined in serial sections.

    • Main Results:

    • Alpha(2)beta(1) integrin was broadly expressed across most cells in the interface tissue.
    • CD11b was predominantly expressed on macrophages and giant cells.
    • CD11a was mainly found on perivascular T lymphocytes, with limited expression of alpha(4)beta(1) and vascular adhesion molecule-1.

    Conclusions:

    • The broad expression of alpha(2)beta(1) suggests a general role in cell adhesion within the interface tissue.
    • Macrophages and T lymphocytes exhibit distinct integrin expression profiles.
    • The co-expression of CD11a, CD11b, and intercellular adhesion molecule-1 suggests that macrophages utilize beta(2) integrins for transmigration in this inflammatory environment.