Inexpensive automation of the Leitz orthoplan microfluorometer using pneumatic components

An automated version of the Leitz Orthoplan MPV I measuring microscope is described. Air powered cylinders are used to place shutters in the main light paths and to move mirrors. Valves connected to the cylinders are sequentially activated by a motor driven shaft provided with cams, which also activates a microswitch connected to a digital voltmeter. Thus a fixed time relationship is obtained between exposure to excitation light and measurement of photomultiplier output. The system described offers a cheap and highly reliable method for fast automatic microfluorometry.