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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
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A Collaborative Study Comparing Three ELISA Systems for Detecting Staphylococcus aureus Enterotoxin A in Sausage

H Schönwälder1, J J Haaijman1, R Holbrook1

  • 1Unilever Research Laboratorium Vlaardingen, P.O. Box, 3130 AC Vlaardingen, The Netherlands.

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|April 18, 2019
PubMed
Summary
This summary is machine-generated.

Monoclonal antibody ELISA (MCA ELISA) offers reliable staphylococcal enterotoxin A (SEA) detection in food quality control. While quantitative polyclonal antibody ELISA (PCA ELISA) and qualitative FEY ELISA also showed promise, MCA ELISA provided superior sensitivity and specificity.

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Area of Science:

  • Food safety and analytical chemistry
  • Microbiology and immunology

Background:

  • Staphylococcal enterotoxin A (SEA) is a significant foodborne pathogen.
  • Accurate detection methods are crucial for food quality control.

Purpose of the Study:

  • To evaluate three Enzyme-Linked Immunosorbent Assays (ELISAs) for staphylococcal enterotoxin A (SEA) detection.
  • To assess their suitability for food quality control laboratories.

Main Methods:

  • Collaborative testing of three ELISAs (two quantitative, one qualitative) in five laboratories.
  • Evaluation using spiked and unspiked ripened and unripened sausage samples.
  • Analysis of sensitivity, reliability, and background responses.

Main Results:

  • Quantitative monoclonal antibody ELISA (MCA ELISA) demonstrated higher sensitivity and reliability with lower background responses compared to polyclonal antibody ELISA (PCA ELISA).
  • MCA ELISA showed lower enterotoxin recovery, particularly in unripened sausage, but yielded more dependable results at low SEA concentrations (1 μg/100 g).
  • The qualitative FEY ELISA provided adequate and reliable results for controlling raw materials and food products.

Conclusions:

  • MCA ELISA is a reliable and sensitive method for SEA detection in food quality control.
  • FEY ELISA is suitable for routine screening of suspicious food samples.
  • Further optimization may be needed for MCA ELISA's enterotoxin recovery in certain food matrices.