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Updated: Oct 6, 2026

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay
Published on: June 2, 2023
A new family of high-affinity ABC manganese and zinc permeases
1Laboratoire de Microbiologie et Génétique Moléculaire UMR5 100, CNRS-Université Paul Sabatier, Toulouse, France. claverys@ibcg.biotoul.fr
Abstract:
Phylogenetic analysis of 47 extracellular putative metal binding receptors (MBRs) belonging to the newly defined cluster suggests the existence of two subclusters. The question of substrate specificity of the corresponding ATP binding cassette (ABC) permeases is discussed, based on data collected from 19 of them concerning their regulation, metal requirement of permease mutants, metal uptake and metal binding. The proposal that the two subclusters correspond to paralogous metal permeases dedicated primarily to manganese and to zinc transport is made. The question of a direct role of MBRs as adhesins of Gram-positive bacteria is then discussed and the importance of metal permeases for cellular processes and host-bacteria interactions is reviewed.
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