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Restriction enzyme BstZ17I is sensitive to cytosine methylation

¹Department of Animal Development and Genetics, Norbyvägen 18A, s-75236, Evolutionary Biology Centrum, Uppsala University, Uppsala, Sweden. kanduri.chandrasekhar@ebc.uu.se

FEMS Microbiology Letters

|June 27, 2001

Abstract:

The cleavage patterns of a subset of restriction enzymes are blocked or impaired when a methylated CpG is overlapped with either the 5' or 3' end of the canonical restriction site. BstZ17I restriction endonuclease is a blunt-end cutter, which recognises the hexanucleotide sequence GTA(downward arrow)TAC. In this report, I show that the BstZ17I restriction enzyme is sensitive to cytosine methylation. Using both in vitro-methylated episomal plasmids and lambdaDNA, I demonstrate that the BstZ17I restriction enzyme is sensitive to cytosine methylation that occurs 3' and/or 5' of the canonical recognition sequence.

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