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Updated: Jul 10, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Development of a quantitative competitive PCR assay for detection and quantification of Escherichia coli O157:H7
1Department of Food Science and Technology, Mississippi State University, Mississippi State, Mississippi 39762-9805, USA.
Abstract:
A quantitative competitive PCR (QC-PCR) assay was developed to detect and quantify Escherichia coli O157:H7 cells. From 10(3) to 10(8) CFU of E. coli O157:H7 cells/ml was quantified in broth or skim milk, and cell densities predicted by QC-PCR were highly related to viable cell counts (r(2) = 0.99 and 0.93, respectively). QC-PCR has potential for quantitative detection of pathogenic bacteria in foods.
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