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Updated: Oct 5, 2026

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
Published on: February 26, 2010
On the Use of Pattern Fluorescence Photobleaching Recovery with Modulation Detection to Obtain Colloidal Size
Brian Fong1, Wieslaw Stryjewski, Paul S. Russo
1Department of Chemistry and Macromolecular Studies Group, Louisiana State University, Baton Rouge, Louisiana, 70803
Abstract:
Fluorescence photobleaching recovery, long a staple tool of complex fluid experimentation, is discussed as a method for size characterization of colloidal particles. Specific comparisons are made to dynamic light scattering, which is often used for sizing particles despite some important limitations. The capabilities of fluorescence photobleaching recovery for size determination are demonstrated for aggregating core-shell silica-polypeptide composite particles suspended in polar organic solvents. Copyright 2001 Academic Press.
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