Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental investigation on mechanical properties of iron metal matrix reinforced with MAX phase V<sub>2</sub>AlC-GO.

Scientific reports·2026
Same author

Delirium secondary to inadvertent administration of naltrexone in patient with opioid dependence syndrome: a case report.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2024
Same author

Paediatric bipolar disorder with Duchenne muscular dystrophy: a case report.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2024
Same author

Cemented Versus Uncemented Hemiarthroplasty for Displaced Intracapsular Neck of Femur Fractures in the Elderly: Outcomes and Costings.

Indian journal of orthopaedics·2024
Same author

Multi-Institutional Clinical Outcomes of Biopsy Gleason Grade Group 5 Prostate Cancers Treated With Contemporary High-Dose Radiation and Long-Term Androgen Deprivation Therapy.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023
Same author

Understanding the effects of chilling on color and quality characteristics of bovine longissimus thoracis.

Meat science·2022

Related Experiment Video

Updated: Jun 24, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 20, 2010

Surface recognition of a protein using designed transition metal complexes.

M A Fazal1, B C Roy, S Sun

  • 1Department of Chemistry, North Dakota State University, Fargo, ND 58105, USA.

Journal of the American Chemical Society
|June 28, 2001
PubMed
Summary

Researchers designed metal complexes to bind specifically to proteins like carbonic anhydrase by matching their surface histidine patterns. This protein recognition method offers new ways for purification, targeting, and sensing.

More Related Videos

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Related Experiment Videos

Last Updated: Jun 24, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 20, 2010

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Area of Science:

  • Biochemistry
  • Bioinorganic Chemistry
  • Protein Chemistry

Background:

  • Proteins possess unique surface histidine residue patterns.
  • Targeting specific proteins is crucial for various biological applications.
  • Carbonic anhydrase (bovine erythrocyte) serves as a model protein.

Purpose of the Study:

  • To design and synthesize transition metal complexes.
  • To achieve strong and selective binding to target proteins based on their surface histidine patterns.
  • To explore applications in protein purification, targeting, and sensing.

Main Methods:

  • Design and synthesis of transition metal complexes.
  • Binding studies in aqueous buffer at pH 7.0.
  • Utilizing the unique surface histidine patterns of proteins for recognition.

Main Results:

  • Achieved strong and selective binding of cupric ions to carbonic anhydrase.
  • Demonstrated successful protein recognition by matching metal ion patterns to protein histidine patterns.
  • Validated the method's efficacy in aqueous buffer conditions.

Conclusions:

  • The developed method enables specific protein recognition based on surface histidine patterns.
  • This approach is applicable to proteins with known structures.
  • The method has broad potential applications in biotechnology and diagnostics.