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Expression analysis of the histamine H(3) receptor in developing rat tissues
A Héron1, A Rouleau, V Cochois
1Laboratoire de Physiologie, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006, Paris, France. anne.heron@pharmacie.univ-paris5.fr
Mechanisms of Development
|June 29, 2001
Summary
Endogenous histamine and its H3 receptor play a role in tissue growth and cell proliferation. Studies show H3 receptor mRNA expression and its activation enhances MAP kinase activity, supporting its mitogenic role.
Area of Science:
- Molecular Biology
- Cell Biology
- Histamine Receptor Research
Background:
- Endogenous histamine is recognized for its role in tissue growth and cell proliferation.
- The H3 receptor's potential function in mediating these mitogenic effects has been hypothesized.
Purpose of the Study:
- To investigate the expression of H3 receptors and histamine synthesis in embryonic tissues.
- To determine if H3 receptor activation influences mitogenic pathways, specifically MAP kinase activity.
Main Methods:
- Analysis of H3-receptor messenger RNA (mRNA) and histamine-synthesizing enzyme expression in embryonic liver, adipose tissue, and epithelia.
- Activation of recombinant H3 receptors in vitro.
- Measurement of MAP kinase activity following H3 receptor activation.
Main Results:
- H3-receptor mRNAs were found to be co-expressed with histamine-synthesizing enzyme mRNAs in embryonic liver, adipose tissue, and various epithelia.
- Activation of recombinant H3 receptors led to enhanced MAP kinase activity.
Conclusions:
- The findings support a role for the H3 receptor in the mitogenic effects of endogenous histamine.
- H3 receptor signaling appears to involve the enhancement of MAP kinase activity, a key pathway in cell proliferation.