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Related Experiment Videos

Changes in gene expression during programmed cell death in tomato cell suspensions.

F A Hoeberichts1, D Orzaez, L H van der Plas

  • 1Agrotechnological Research Institute (ATO), Wageningen University and Research Centre, The Netherlands.

Plant Molecular Biology
|June 30, 2001
PubMed
Summary
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Researchers identified novel genes involved in plant programmed cell death (PCD) using tomato cell cultures. Camptothecin treatment triggered PCD, revealing changes in DAD1, HSR203, and several new gene expressions, offering insights into plant cell death mechanisms.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Programmed cell death (PCD) is crucial for plant development and defense.
  • Understanding the genetic regulation of plant PCD is essential for agricultural and biological research.
  • Tomato cell cultures provide a tractable model system for studying plant PCD.

Purpose of the Study:

  • To identify genes involved in plant programmed cell death (PCD).
  • To characterize gene expression changes during camptothecin-induced PCD in tomato cells.
  • To discover novel genes participating in plant cell death pathways.

Main Methods:

  • Suspension-cultured tomato cells were treated with camptothecin to induce PCD.
  • Internucleosomal DNA degradation was assessed to confirm apoptosis-like cell death.

Related Experiment Videos

  • Differential display and gene isolation were employed to identify differentially expressed genes.
  • Homology searches were performed on isolated cDNA clones.

    • Main Results:

    • Camptothecin induced PCD with DNA fragmentation, similar to animal apoptosis.
    • Expression of tomato DAD1 (programmed cell death) decreased, while HSR203 increased.
    • Two novel up-regulated genes (CTU1, CTU2) and four down-regulated genes (CTD1, CTD2, CTD4, CTD5) were identified.
    • CTU1 showed homology to glutathione S-transferases; CTD1 to Aux/IAA genes.

    Conclusions:

    • The study identified several novel candidate genes involved in plant PCD.
    • Gene expression patterns provide insights into the molecular mechanisms of plant cell death.
    • Calcium channel blockers like lanthanum chloride can inhibit camptothecin-induced cell death, suggesting a role for calcium signaling.