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IgG reactivity against citrullinated myelin basic protein in multiple sclerosis
J de Seze1, S Dubucquoi, D Lefranc
1Department of Immunology, CHRU of Lille, Lille, France. j-deseze@chru-lille.fr
Journal of Neuroimmunology
|June 30, 2001
Summary
Researchers investigated immune responses to citrullinated myelin basic protein (MBP) in multiple sclerosis (MS). While some MBP epitopes showed reactivity, citrullination did not significantly alter antibody responses in MS patients compared to controls.
Area of Science:
- Neuroimmunology
- Biochemistry
Background:
- Elevated citrullinated myelin basic protein (MBP-C8) is observed in multiple sclerosis (MS) brains.
- The role of immune responses to post-translationally modified MBP in MS pathogenesis is not fully understood.
Purpose of the Study:
- To compare immunoglobulin G (IgG) antibody levels against various myelin basic protein (MBP) epitopes, both native and citrullinated, in the cerebrospinal fluid (CSF) and sera of MS patients versus controls.
- To investigate the impact of MBP citrullination on antibody reactivity in MS.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to analyze antibody reactivity.
- Antibody responses to five specific MBP peptides (native and citrullinated) were assessed in 60 MS patients and 30 other neurological disease (OND) controls.
- Peptides included MBP(75-98), native and citrullinated MBP(108-126), and native and citrullinated MBP(151-170).
Main Results:
- All tested peptides elicited immune reactivity in both MS and OND patient samples.
- A significantly higher reactivity against native MBP(151-170) (peptide 4) was observed in the CSF of MS patients compared to OND patients.
- The citrullination state of MBP(108-126) (peptide 2) significantly altered immune reactivity patterns more in MS patients than in OND patients.
Conclusions:
- While certain MBP epitopes may be targets in MS, this study found no significant difference in antibody responses to citrullinated MBP peptides between MS patients and controls.
- The citrullination status of specific MBP epitopes can differentially affect immune reactivity patterns in MS patients, warranting further investigation.