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Updated: Aug 4, 2026
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
Published on: December 14, 2015
Genetic material is present in cadaveric dermis and cadaveric fascia lata
1Urodynamics and Continence Center, Division of Urology, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Purpose:
We determined whether genetic material is present in the commercially processed cadaveric allografts used in sling surgery.
Materials And Methods:
We evaluated 16 samples from 2 commercial sources of human allograft, including 8 each of freeze-dried gamma irradiated cadaveric fascia lata and acellular cadaveric dermis. Fresh human rectus fascia and sterile saline served as positive and negative controls, respectively. All samples underwent a standard proteinase K/sodium dodecyl sulfate/phenol extraction technique to isolate DNA. Polymerase chain reaction was done to amplify the retrieved DNA material, spectrophotometry to quantify DNA concentration and agarose gel electrophoresis to determine the size of DNA fragments.
Results:
Of the 16 samples tested from 2 commercial sources of human allograft fascia 14 (87.5%) contained DNA. Mean DNA concentration plus or minus standard error was 258.3 +/- 80.1 and 272.8 +/- 168.8 microg./gm. tissue for cadaveric fascia lata and cadaveric dermis, respectively. Polymerase chain reaction amplified DNA segments of 2,000 bp from 1 of each of the 8 samples of cadaveric fascia lata and cadaveric dermis.
Conclusions:
Freeze-dried gamma irradiated cadaveric fascia lata and acellular cadaveric dermis contained intact DNA.
