Related Experiment Video
Updated: Aug 19, 2026

An Orthotopic Murine Model of Human Prostate Cancer Metastasis
Published on: September 19, 2013
Distinct effects of PIAS proteins on androgen-mediated gene activation in prostate cancer cells
1Division of Hematology-Oncology, Department of Medicine, University of California, Los Angeles, California, CA 90095, USA.
Abstract:
Androgen signaling influences the development and growth of prostate carcinoma. The transcriptional activity of androgen receptor (AR) is regulated by positive or negative transcriptional cofactors. We report here that PIAS1, PIAS3, and PIASy of the protein inhibitor of activated STAT (PIAS) family, which are expressed in human prostate, display distinct effects on AR-mediated gene activation in prostate cancer cells. While PIAS1 and PIAS3 enhance the transcriptional activity of AR, PIASy acts as a potent inhibitor of AR in prostate cancer cells. The effects of PIAS proteins on AR are competitive. PIASy binds to AR but does not affect the DNA binding activity of AR. An NH2-terminal LXXLL signature motif of PIASy, although not required for PIASy-AR interaction, is essential for the transrepression activity of PIASy. Our results identify PIASy as a transcriptional corepressor of AR and suggest that different PIAS proteins have distinct effects on AR signaling in prostate cancer cells.
Related Concept Videos
Abnormal Proliferation
The Ras Gene
Ras is a superfamily...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Amplifying Signals via Enzymatic Cascade
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway

