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Cell surface changes in mouse peritoneal macrophages after induction with proteose peptone or thioglycollate
Abstract:
Some five to six per cent of mouse PEC spontaneously form rosettes with SRBC. This weak intercellular interaction is most likely mediated by a "receptor" for SRBC located on the surface of peritoneal macrophages. Manifestation of the receptor is influenced by the genetic background and the H-2 haplotype of PEC donors; a high proportion of RFC is associated with the H-2s haplotype. PEC derived from intact mice markedly differ in morphological and functional characteristics from those of PP- or TG-pretreated donors. Formation of rosettes by induced macrophages depends on the time interval between stimulation and PEC harvesting and on the type of the inducing agent; it is also radiosensitive and more responsive to the action of colchicine. TG-induced macrophages have a significantly reduced capacity to bind syngeneic lymphoid cells. The difference in adhesivity between intact and stimulated PEC can be abolished by glutaraldehyde prefixation. In vivo induction results in modified morphological and functional properties of macrophages, including transformation of their cell surface.
Insights
Mouse peritoneal exudate cells (PECs) can form rosettes with sheep red blood cells (SRBCs), a process influenced by genetics and macrophage stimulation. This interaction reveals insights into macrophage surface receptor expression and function.
Area of Science:
- Immunology
- Cell Biology
Background:
- Peritoneal exudate cells (PECs) from mice exhibit spontaneous rosette formation with sheep red blood cells (SRBCs).
- This interaction is mediated by SRBC receptors on peritoneal macrophages and is influenced by genetic factors, including the H-2 haplotype.
Purpose of the Study:
- To investigate the factors affecting rosette formation by mouse PECs.
- To characterize the differences between PECs from intact and stimulated mice.
Main Methods:
- Assessing spontaneous rosette formation of PECs with SRBCs.
- Analyzing the influence of genetic background (H-2 haplotype) and stimulation (PP- or TG-pretreatment) on PECs.
- Evaluating the effects of time, inducing agents, radiation, colchicine, and glutaraldehyde on rosette formation.
Main Results:
- A high proportion of rosette-forming cells (RFCs) is associated with the H-2s haplotype.
- Stimulated PECs differ morphologically and functionally from intact PECs.
- Macrophage rosette formation is time-dependent, radiosensitive, and affected by colchicine; TG-induced macrophages show reduced binding of syngeneic lymphoid cells.
Conclusions:
- Macrophage receptor expression for SRBCs is genetically controlled and modulated by in vivo stimulation.
- Macrophage surface properties and adhesivity are altered following in vivo induction, impacting cell interactions.