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Related Experiment Videos

Terminal deoxynucleotidyl transferase-positive cells in human tonsils.

J A Strauchen1, L K Miller

  • 1Dept of Pathology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029, USA.

American Journal of Clinical Pathology
|July 13, 2001
PubMed
Summary

Normal human tonsils contain terminal deoxynucleotidyl transferase (TdT)-positive cells, indicating tonsils are sites of lymphopoiesis. These TdT-positive precursor cells may contribute to indolent T-lymphoblastic proliferations in the tonsils.

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Area of Science:

  • Immunology
  • Cell Biology
  • Pathology

Background:

  • Recent recognition of indolent TdT-positive T-lymphoblastic proliferations in tonsils and oropharynx.
  • Uncertainty regarding the cellular origin of these proliferations.

Purpose of the Study:

  • To investigate the presence and phenotype of TdT-positive cells in normal human tonsils.
  • To determine if tonsils are a site of lymphopoiesis.

Main Methods:

  • Immunohistochemical staining of normal human tonsils from children and adults.
  • Double-antibody staining to determine cell phenotype.
  • Comparison with reactive lymph nodes as controls.

Main Results:

  • TdT-positive cells were readily found in tonsils, distributed in discrete foci.

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  • These cells exhibited the phenotype of uncommitted early lymphoid precursors (CD3-, CD79a-, CD10-).
  • TdT-positive foci were absent in control reactive lymph nodes.

    • Conclusions:

    • Human tonsils harbor TdT-positive lymphoid precursor cells, similar to bone marrow and thymus.
    • Tonsils are identified as sites of lymphopoiesis.
    • The presence of these precursor cells may play a role in the pathogenesis of tonsillar T-lymphoblastic proliferations.